Construct: shRNA TRCN0000427061

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026055.1-215s21c1
DNA Barcode:
GATTCACACAATGGCAAGACT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rpl39 (67248)
Gene Description:
ribosomal protein L39
Transcript:
RefSeq NM_026055.1 (NON-CURRENT)
Match location:
Position 215 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427061)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTCACACAATGGCAAGACT
Hairpin Sequence:
5'-CCGG-GATTCACACAATGGCAAGACT-CTCGAG-AGTCTTGCCATTGTGTGAATC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTCACACAATGGCAAGACTCTCGAGAGTCTTGCCATTGTGTGAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTCACACAATGGCAAGACTCTCGAGAGTCTTGCCATTGTGTGAATC-3'

Other clones with same target sequence:

(none)