Construct: shRNA TRCN0000427135

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004841.3-1634s21c1
DNA Barcode:
ATCTCGGACTCTAACTCTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RASAL2 (9462)
Gene Description:
RAS protein activator like 2
Transcript:
RefSeq NM_004841.3 (NON-CURRENT)
Match location:
Position 1634 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427135)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCTCGGACTCTAACTCTTAT
Hairpin Sequence:
5'-CCGG-ATCTCGGACTCTAACTCTTAT-CTCGAG-ATAAGAGTTAGAGTCCGAGAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCTCGGACTCTAACTCTTATCTCGAGATAAGAGTTAGAGTCCGAGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCTCGGACTCTAACTCTTATCTCGAGATAAGAGTTAGAGTCCGAGAT-3'

Other clones with same target sequence:

(none)