Construct: shRNA TRCN0000427141

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006076.4-1451s21c1
DNA Barcode:
GACTTAGGATCAGCCAAGTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AGFG2 (3268)
Gene Description:
ArfGAP with FG repeats 2
Transcript:
RefSeq NM_006076.4 (NON-CURRENT)
Match location:
Position 1451 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427141)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTTAGGATCAGCCAAGTTG
Hairpin Sequence:
5'-CCGG-GACTTAGGATCAGCCAAGTTG-CTCGAG-CAACTTGGCTGATCCTAAGTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTTAGGATCAGCCAAGTTGCTCGAGCAACTTGGCTGATCCTAAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTTAGGATCAGCCAAGTTGCTCGAGCAACTTGGCTGATCCTAAGTC-3'

Other clones with same target sequence:

(none)