Construct: shRNA TRCN0000427221

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002246.2-522s21c1
DNA Barcode:
TCACGCTCGTCATGTTCCAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNK3 (3777)
Gene Description:
potassium two pore domain channel subfamily K member 3
Transcript:
RefSeq NM_002246.2 (NON-CURRENT)
Match location:
Position 522 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427221)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCACGCTCGTCATGTTCCAGA
Hairpin Sequence:
5'-CCGG-TCACGCTCGTCATGTTCCAGA-CTCGAG-TCTGGAACATGACGAGCGTGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCACGCTCGTCATGTTCCAGACTCGAGTCTGGAACATGACGAGCGTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCACGCTCGTCATGTTCCAGACTCGAGTCTGGAACATGACGAGCGTGA-3'

Other clones with same target sequence:

(none)