Construct: shRNA TRCN0000427265

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004036.3-2705s21c1
DNA Barcode:
GTGCCTTCCAAGTACTCTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADCY3 (109)
Gene Description:
adenylate cyclase 3
Transcript:
RefSeq NM_004036.3 (NON-CURRENT)
Match location:
Position 2705 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427265)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGCCTTCCAAGTACTCTATG
Hairpin Sequence:
5'-CCGG-GTGCCTTCCAAGTACTCTATG-CTCGAG-CATAGAGTACTTGGAAGGCAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGCCTTCCAAGTACTCTATGCTCGAGCATAGAGTACTTGGAAGGCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGCCTTCCAAGTACTCTATGCTCGAGCATAGAGTACTTGGAAGGCAC-3'

Other clones with same target sequence:

(none)