Construct: shRNA TRCN0000427286

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020132.4-1278s21c1
DNA Barcode:
TCGCAGACTGATAGGAGTAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AGPAT3 (56894)
Gene Description:
1-acylglycerol-3-phosphate O-acyltransferase 3
Transcript:
RefSeq NM_020132.4 (NON-CURRENT)
Match location:
Position 1278 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427286)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGCAGACTGATAGGAGTAAC
Hairpin Sequence:
5'-CCGG-TCGCAGACTGATAGGAGTAAC-CTCGAG-GTTACTCCTATCAGTCTGCGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGCAGACTGATAGGAGTAACCTCGAGGTTACTCCTATCAGTCTGCGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGCAGACTGATAGGAGTAACCTCGAGGTTACTCCTATCAGTCTGCGA-3'

Other clones with same target sequence:

(none)