Construct: shRNA TRCN0000427318

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032204.3-855s21c1
DNA Barcode:
TGATACCTGCACCACACTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ASCC2 (84164)
Gene Description:
activating signal cointegrator 1 complex subunit 2
Transcript:
RefSeq NM_032204.3 (NON-CURRENT)
Match location:
Position 855 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427318)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATACCTGCACCACACTTTG
Hairpin Sequence:
5'-CCGG-TGATACCTGCACCACACTTTG-CTCGAG-CAAAGTGTGGTGCAGGTATCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATACCTGCACCACACTTTGCTCGAGCAAAGTGTGGTGCAGGTATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATACCTGCACCACACTTTGCTCGAGCAAAGTGTGGTGCAGGTATCA-3'

Other clones with same target sequence:

(none)