Construct: shRNA TRCN0000427394

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024691.3-300s21c1
DNA Barcode:
AGACCATGAGGAGGGCTATGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF419 (79744)
Gene Description:
zinc finger protein 419
Transcript:
RefSeq NM_024691.3 (NON-CURRENT)
Match location:
Position 300 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427394)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACCATGAGGAGGGCTATGT
Hairpin Sequence:
5'-CCGG-AGACCATGAGGAGGGCTATGT-CTCGAG-ACATAGCCCTCCTCATGGTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACCATGAGGAGGGCTATGTCTCGAGACATAGCCCTCCTCATGGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACCATGAGGAGGGCTATGTCTCGAGACATAGCCCTCCTCATGGTCT-3'

Other clones with same target sequence:

(none)