Construct: shRNA TRCN0000427438

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003490.3-461s21c1
DNA Barcode:
ATACAGACTGGTCGAAGTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SYN3 (8224)
Gene Description:
synapsin III
Transcript:
RefSeq NM_003490.3 (NON-CURRENT)
Match location:
Position 461 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427438)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATACAGACTGGTCGAAGTATT
Hairpin Sequence:
5'-CCGG-ATACAGACTGGTCGAAGTATT-CTCGAG-AATACTTCGACCAGTCTGTAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATACAGACTGGTCGAAGTATTCTCGAGAATACTTCGACCAGTCTGTATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATACAGACTGGTCGAAGTATTCTCGAGAATACTTCGACCAGTCTGTAT-3'

Other clones with same target sequence:

(none)