Construct: shRNA TRCN0000427469

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032175.2-2059s21c1
DNA Barcode:
GAGTGTTCTGTATCAACATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UTP15 (84135)
Gene Description:
UTP15 small subunit processome component
Transcript:
RefSeq NM_032175.2 (NON-CURRENT)
Match location:
Position 2059 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427469)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTGTTCTGTATCAACATTT
Hairpin Sequence:
5'-CCGG-GAGTGTTCTGTATCAACATTT-CTCGAG-AAATGTTGATACAGAACACTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTGTTCTGTATCAACATTTCTCGAGAAATGTTGATACAGAACACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTGTTCTGTATCAACATTTCTCGAGAAATGTTGATACAGAACACTC-3'

Other clones with same target sequence:

(none)