Construct: shRNA TRCN0000427536

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000733.3-635s21c1
DNA Barcode:
GTGATGTCGGTGGCCACAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CD3E (916)
Gene Description:
CD3e molecule
Transcript:
RefSeq NM_000733.3 (NON-CURRENT)
Match location:
Position 635 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427536)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGATGTCGGTGGCCACAATT
Hairpin Sequence:
5'-CCGG-GTGATGTCGGTGGCCACAATT-CTCGAG-AATTGTGGCCACCGACATCAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGATGTCGGTGGCCACAATTCTCGAGAATTGTGGCCACCGACATCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGATGTCGGTGGCCACAATTCTCGAGAATTGTGGCCACCGACATCAC-3'

Other clones with same target sequence:

(none)