Construct: shRNA TRCN0000427543

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152784.3-207s21c1
DNA Barcode:
CTACAACAACACGCTTGATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CATSPERD (257062)
Gene Description:
cation channel sperm associated auxiliary subunit delta
Transcript:
RefSeq NM_152784.3 (NON-CURRENT)
Match location:
Position 207 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427543)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACAACAACACGCTTGATTA
Hairpin Sequence:
5'-CCGG-CTACAACAACACGCTTGATTA-CTCGAG-TAATCAAGCGTGTTGTTGTAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACAACAACACGCTTGATTACTCGAGTAATCAAGCGTGTTGTTGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACAACAACACGCTTGATTACTCGAGTAATCAAGCGTGTTGTTGTAG-3'

Other clones with same target sequence:

(none)