Construct: shRNA TRCN0000427554

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138403.4-746s21c1
DNA Barcode:
TTCAGTGAGGAGGAGGTCAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYL10 (93408)
Gene Description:
myosin light chain 10
Transcript:
RefSeq NM_138403.4 (NON-CURRENT)
Match location:
Position 746 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427554)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCAGTGAGGAGGAGGTCAAG
Hairpin Sequence:
5'-CCGG-TTCAGTGAGGAGGAGGTCAAG-CTCGAG-CTTGACCTCCTCCTCACTGAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCAGTGAGGAGGAGGTCAAGCTCGAGCTTGACCTCCTCCTCACTGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCAGTGAGGAGGAGGTCAAGCTCGAGCTTGACCTCCTCCTCACTGAA-3'

Other clones with same target sequence:

(none)