Construct: shRNA TRCN0000427597

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008487.3-2367s21c1
DNA Barcode:
ACAGCCATTGATCAATCTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arhgef2 (16800)
Gene Description:
rho/rac guanine nucleotide exchange factor (GEF) 2
Transcript:
RefSeq NM_008487.3 (CURRENT)
Match location:
Position 2367 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427597)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAGCCATTGATCAATCTTTA
Hairpin Sequence:
5'-CCGG-ACAGCCATTGATCAATCTTTA-CTCGAG-TAAAGATTGATCAATGGCTGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAGCCATTGATCAATCTTTACTCGAGTAAAGATTGATCAATGGCTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAGCCATTGATCAATCTTTACTCGAGTAAAGATTGATCAATGGCTGT-3'

Other clones with same target sequence:

(none)