Construct: shRNA TRCN0000427618

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005258.2-160s21c1
DNA Barcode:
ACAGTCGGATCCAGAGCTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GCHFR (2644)
Gene Description:
GTP cyclohydrolase I feedback regulator
Transcript:
RefSeq NM_005258.2 (NON-CURRENT)
Match location:
Position 160 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427618)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAGTCGGATCCAGAGCTGAT
Hairpin Sequence:
5'-CCGG-ACAGTCGGATCCAGAGCTGAT-CTCGAG-ATCAGCTCTGGATCCGACTGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAGTCGGATCCAGAGCTGATCTCGAGATCAGCTCTGGATCCGACTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAGTCGGATCCAGAGCTGATCTCGAGATCAGCTCTGGATCCGACTGT-3'

Other clones with same target sequence:

(none)