Construct: shRNA TRCN0000427633

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016732.1-1390s21c1
DNA Barcode:
GAGTTACTGGCCTACTCCTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RALY (22913)
Gene Description:
RALY heterogeneous nuclear ribonucleoprotein
Transcript:
RefSeq NM_016732.1 (NON-CURRENT)
Match location:
Position 1390 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427633)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTTACTGGCCTACTCCTTC
Hairpin Sequence:
5'-CCGG-GAGTTACTGGCCTACTCCTTC-CTCGAG-GAAGGAGTAGGCCAGTAACTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTTACTGGCCTACTCCTTCCTCGAGGAAGGAGTAGGCCAGTAACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTTACTGGCCTACTCCTTCCTCGAGGAAGGAGTAGGCCAGTAACTC-3'

Other clones with same target sequence:

(none)