Construct: shRNA TRCN0000427655

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017653.3-2486s21c1
DNA Barcode:
AGAGGATTGCACGAGTGTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DYM (54808)
Gene Description:
dymeclin
Transcript:
RefSeq NM_017653.3 (NON-CURRENT)
Match location:
Position 2486 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427655)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGGATTGCACGAGTGTGTT
Hairpin Sequence:
5'-CCGG-AGAGGATTGCACGAGTGTGTT-CTCGAG-AACACACTCGTGCAATCCTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGGATTGCACGAGTGTGTTCTCGAGAACACACTCGTGCAATCCTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGGATTGCACGAGTGTGTTCTCGAGAACACACTCGTGCAATCCTCT-3'

Other clones with same target sequence:

(none)