Construct: shRNA TRCN0000427662

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002751.5-364s21c1
DNA Barcode:
GTACTTGGTGACCACCCTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAPK11 (5600)
Gene Description:
mitogen-activated protein kinase 11
Transcript:
RefSeq NM_002751.5 (NON-CURRENT)
Match location:
Position 364 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427662)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTACTTGGTGACCACCCTGAT
Hairpin Sequence:
5'-CCGG-GTACTTGGTGACCACCCTGAT-CTCGAG-ATCAGGGTGGTCACCAAGTAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTACTTGGTGACCACCCTGATCTCGAGATCAGGGTGGTCACCAAGTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTACTTGGTGACCACCCTGATCTCGAGATCAGGGTGGTCACCAAGTAC-3'

Other clones with same target sequence:

(none)