Construct: shRNA TRCN0000427665

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032814.3-1788s21c1
DNA Barcode:
CAGATGGGATGGTTTAGTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RNFT2 (84900)
Gene Description:
ring finger protein, transmembrane 2
Transcript:
RefSeq NM_032814.3 (NON-CURRENT)
Match location:
Position 1788 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427665)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGATGGGATGGTTTAGTTTA
Hairpin Sequence:
5'-CCGG-CAGATGGGATGGTTTAGTTTA-CTCGAG-TAAACTAAACCATCCCATCTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGATGGGATGGTTTAGTTTACTCGAGTAAACTAAACCATCCCATCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGATGGGATGGTTTAGTTTACTCGAGTAAACTAAACCATCCCATCTG-3'

Other clones with same target sequence:

(none)