Construct: shRNA TRCN0000427718

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033514.3-898s21c1
DNA Barcode:
GTCCTGAGGAAGATTACTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LIMS3 (96626)
Gene Description:
LIM zinc finger domain containing 3
Transcript:
RefSeq NM_033514.3 (NON-CURRENT)
Match location:
Position 898 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427718)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCCTGAGGAAGATTACTATT
Hairpin Sequence:
5'-CCGG-GTCCTGAGGAAGATTACTATT-CTCGAG-AATAGTAATCTTCCTCAGGAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCCTGAGGAAGATTACTATTCTCGAGAATAGTAATCTTCCTCAGGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCCTGAGGAAGATTACTATTCTCGAGAATAGTAATCTTCCTCAGGAC-3'

Other clones with same target sequence:

(none)