Construct: shRNA TRCN0000427733

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014286.3-908s21c1
DNA Barcode:
GGCTACAGCCCTCTGCATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NCS1 (23413)
Gene Description:
neuronal calcium sensor 1
Transcript:
RefSeq NM_014286.3 (NON-CURRENT)
Match location:
Position 908 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427733)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCTACAGCCCTCTGCATAAA
Hairpin Sequence:
5'-CCGG-GGCTACAGCCCTCTGCATAAA-CTCGAG-TTTATGCAGAGGGCTGTAGCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCTACAGCCCTCTGCATAAACTCGAGTTTATGCAGAGGGCTGTAGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCTACAGCCCTCTGCATAAACTCGAGTTTATGCAGAGGGCTGTAGCC-3'

Other clones with same target sequence:

(none)