Construct: shRNA TRCN0000427761

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007001.2-398s21c1
DNA Barcode:
GGATTATCAAGCACAAGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC35D2 (11046)
Gene Description:
solute carrier family 35 member D2
Transcript:
RefSeq NM_007001.2 (NON-CURRENT)
Match location:
Position 398 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427761)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGATTATCAAGCACAAGTAAA
Hairpin Sequence:
5'-CCGG-GGATTATCAAGCACAAGTAAA-CTCGAG-TTTACTTGTGCTTGATAATCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGATTATCAAGCACAAGTAAACTCGAGTTTACTTGTGCTTGATAATCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGATTATCAAGCACAAGTAAACTCGAGTTTACTTGTGCTTGATAATCC-3'

Other clones with same target sequence:

(none)