Construct: shRNA TRCN0000427816
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_003284.3-217s21c1
- DNA Barcode:
- TGCGCTTCACACAGCACCAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TNP1 (7141)
- Gene Description:
- transition protein 1
- Transcript:
- RefSeq NM_003284.3 (NON-CURRENT)
- Match location:
- Position 217 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000427816)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TGCGCTTCACACAGCACCAAG
- Hairpin Sequence:
-
5'-CCGG-TGCGCTTCACACAGCACCAAG-CTCGAG-CTTGGTGCTGTGTGAAGCGCA-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTGCGCTTCACACAGCACCAAGCTCGAGCTTGGTGCTGTGTGAAGCGCATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATGCGCTTCACACAGCACCAAGCTCGAGCTTGGTGCTGTGTGAAGCGCA-3'
Other clones with same target sequence:
(none)
