Construct: shRNA TRCN0000427825

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080722.3-2501s21c1
DNA Barcode:
CCCTTATCGGGAGCAACAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADAMTS14 (140766)
Gene Description:
ADAM metallopeptidase with thrombospondin type 1 motif 14
Transcript:
RefSeq NM_080722.3 (NON-CURRENT)
Match location:
Position 2501 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427825)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTATCGGGAGCAACAATG
Hairpin Sequence:
5'-CCGG-CCCTTATCGGGAGCAACAATG-CTCGAG-CATTGTTGCTCCCGATAAGGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTATCGGGAGCAACAATGCTCGAGCATTGTTGCTCCCGATAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTATCGGGAGCAACAATGCTCGAGCATTGTTGCTCCCGATAAGGG-3'

Other clones with same target sequence:

(none)