Construct: shRNA TRCN0000427851

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014585.5-2452s21c1
DNA Barcode:
TTGTTCAAGACTAGCTAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC40A1 (30061)
Gene Description:
solute carrier family 40 member 1
Transcript:
RefSeq NM_014585.5 (CURRENT)
Match location:
Position 2452 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427851)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGTTCAAGACTAGCTAATTT
Hairpin Sequence:
5'-CCGG-TTGTTCAAGACTAGCTAATTT-CTCGAG-AAATTAGCTAGTCTTGAACAA-TTTTTGGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGTTCAAGACTAGCTAATTTCTCGAGAAATTAGCTAGTCTTGAACAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGTTCAAGACTAGCTAATTTCTCGAGAAATTAGCTAGTCTTGAACAA-3'

Other clones with same target sequence:

(none)