Construct: shRNA TRCN0000427884

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019000.3-1589s21c1
DNA Barcode:
TAAGCATGGAAATGAACTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RETREG1 (54463)
Gene Description:
reticulophagy regulator 1
Transcript:
RefSeq NM_019000.3 (NON-CURRENT)
Match location:
Position 1589 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427884)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAAGCATGGAAATGAACTTTA
Hairpin Sequence:
5'-CCGG-TAAGCATGGAAATGAACTTTA-CTCGAG-TAAAGTTCATTTCCATGCTTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAAGCATGGAAATGAACTTTACTCGAGTAAAGTTCATTTCCATGCTTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAAGCATGGAAATGAACTTTACTCGAGTAAAGTTCATTTCCATGCTTA-3'

Other clones with same target sequence:

(none)