Construct: shRNA TRCN0000428083

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014028.3-509s21c1
DNA Barcode:
TTGTGCCAGAAGTCTCTTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OSTM1 (28962)
Gene Description:
osteoclastogenesis associated transmembrane protein 1
Transcript:
RefSeq NM_014028.3 (NON-CURRENT)
Match location:
Position 509 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000428083)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGTGCCAGAAGTCTCTTAAT
Hairpin Sequence:
5'-CCGG-TTGTGCCAGAAGTCTCTTAAT-CTCGAG-ATTAAGAGACTTCTGGCACAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGTGCCAGAAGTCTCTTAATCTCGAGATTAAGAGACTTCTGGCACAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGTGCCAGAAGTCTCTTAATCTCGAGATTAAGAGACTTCTGGCACAA-3'

Other clones with same target sequence:

(none)