Construct: shRNA TRCN0000428092

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011957.2-598s21c1
DNA Barcode:
CTCAGCTGCCAGTGATCAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Creb3l1 (26427)
Gene Description:
cAMP responsive element binding protein 3-like 1
Transcript:
RefSeq NM_011957.2 (CURRENT)
Match location:
Position 598 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000428092)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAGCTGCCAGTGATCAAAG
Hairpin Sequence:
5'-CCGG-CTCAGCTGCCAGTGATCAAAG-CTCGAG-CTTTGATCACTGGCAGCTGAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAGCTGCCAGTGATCAAAGCTCGAGCTTTGATCACTGGCAGCTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAGCTGCCAGTGATCAAAGCTCGAGCTTTGATCACTGGCAGCTGAG-3'

Other clones with same target sequence:

  1. TRCN0000371900