Construct: shRNA TRCN0000428105

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005497.3-1446s21c1
DNA Barcode:
AGACCTCCGTCTGGATTTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GJC1 (10052)
Gene Description:
gap junction protein gamma 1
Transcript:
RefSeq NM_005497.3 (NON-CURRENT)
Match location:
Position 1446 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000428105)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACCTCCGTCTGGATTTAAT
Hairpin Sequence:
5'-CCGG-AGACCTCCGTCTGGATTTAAT-CTCGAG-ATTAAATCCAGACGGAGGTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACCTCCGTCTGGATTTAATCTCGAGATTAAATCCAGACGGAGGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACCTCCGTCTGGATTTAATCTCGAGATTAAATCCAGACGGAGGTCT-3'

Other clones with same target sequence:

(none)