Construct: shRNA TRCN0000428145

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183113.3-3527s21c1
DNA Barcode:
CAGTTACACTACCACTATTAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
4932414N04Rik (75721)
Gene Description:
RIKEN cDNA 4932414N04 gene
Transcript:
RefSeq NM_183113.3 (CURRENT)
Match location:
Position 3527 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000428145)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTTACACTACCACTATTAG
Hairpin Sequence:
5'-CCGG-CAGTTACACTACCACTATTAG-CTCGAG-CTAATAGTGGTAGTGTAACTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTTACACTACCACTATTAGCTCGAGCTAATAGTGGTAGTGTAACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTTACACTACCACTATTAGCTCGAGCTAATAGTGGTAGTGTAACTG-3'

Other clones with same target sequence:

(none)