Construct: shRNA TRCN0000428243

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153225.3-1335s21c1
DNA Barcode:
GTGACGGAAGTGTCATTTAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SBSPON (157869)
Gene Description:
somatomedin B and thrombospondin type 1 domain containing
Transcript:
RefSeq NM_153225.3 (NON-CURRENT)
Match location:
Position 1335 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000428243)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGACGGAAGTGTCATTTAAG
Hairpin Sequence:
5'-CCGG-GTGACGGAAGTGTCATTTAAG-CTCGAG-CTTAAATGACACTTCCGTCAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGACGGAAGTGTCATTTAAGCTCGAGCTTAAATGACACTTCCGTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGACGGAAGTGTCATTTAAGCTCGAGCTTAAATGACACTTCCGTCAC-3'

Other clones with same target sequence:

(none)