Construct: shRNA TRCN0000428282

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014218.2-1239s21c1
DNA Barcode:
GCCTCTCTCTTGCTTACAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIR2DL1 (3802)
Gene Description:
killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 1
Transcript:
RefSeq NM_014218.2 (NON-CURRENT)
Match location:
Position 1239 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000428282)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCTCTCTTGCTTACAAAT
Hairpin Sequence:
5'-CCGG-GCCTCTCTCTTGCTTACAAAT-CTCGAG-ATTTGTAAGCAAGAGAGAGGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCTCTCTTGCTTACAAATCTCGAGATTTGTAAGCAAGAGAGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCTCTCTTGCTTACAAATCTCGAGATTTGTAAGCAAGAGAGAGGC-3'

Other clones with same target sequence:

(none)