Construct: shRNA TRCN0000428468

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001003683.1-558s21c1
DNA Barcode:
TAAATGGTCTTTCGATGTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDE1A (5136)
Gene Description:
phosphodiesterase 1A
Transcript:
RefSeq NM_001003683.1 (NON-CURRENT)
Match location:
Position 558 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000428468)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAAATGGTCTTTCGATGTATT
Hairpin Sequence:
5'-CCGG-TAAATGGTCTTTCGATGTATT-CTCGAG-AATACATCGAAAGACCATTTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAAATGGTCTTTCGATGTATTCTCGAGAATACATCGAAAGACCATTTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAAATGGTCTTTCGATGTATTCTCGAGAATACATCGAAAGACCATTTA-3'

Other clones with same target sequence:

(none)