Construct: shRNA TRCN0000428503

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010566.2-2586s21c1
DNA Barcode:
GCATATCCTGATCAGCATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Inpp5d (16331)
Gene Description:
inositol polyphosphate-5-phosphatase D
Transcript:
RefSeq NM_010566.2 (NON-CURRENT)
Match location:
Position 2586 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000428503)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATATCCTGATCAGCATTAA
Hairpin Sequence:
5'-CCGG-GCATATCCTGATCAGCATTAA-CTCGAG-TTAATGCTGATCAGGATATGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATATCCTGATCAGCATTAACTCGAGTTAATGCTGATCAGGATATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATATCCTGATCAGCATTAACTCGAGTTAATGCTGATCAGGATATGC-3'

Other clones with same target sequence:

(none)