Construct: shRNA TRCN0000428511

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014606.1-1226s21c1
DNA Barcode:
GCCCGAGCTGATCGCTTTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HERC3 (8916)
Gene Description:
HECT and RLD domain containing E3 ubiquitin protein ligase 3
Transcript:
RefSeq NM_014606.1 (NON-CURRENT)
Match location:
Position 1226 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000428511)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCGAGCTGATCGCTTTAAA
Hairpin Sequence:
5'-CCGG-GCCCGAGCTGATCGCTTTAAA-CTCGAG-TTTAAAGCGATCAGCTCGGGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCGAGCTGATCGCTTTAAACTCGAGTTTAAAGCGATCAGCTCGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCGAGCTGATCGCTTTAAACTCGAGTTTAAAGCGATCAGCTCGGGC-3'

Other clones with same target sequence:

(none)