Construct: shRNA TRCN0000428529

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010712.2-1154s21c1
DNA Barcode:
ACAGTAATCTGGGCATCATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lhx4 (16872)
Gene Description:
LIM homeobox protein 4
Transcript:
RefSeq NM_010712.2 (CURRENT)
Match location:
Position 1154 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000428529)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAGTAATCTGGGCATCATTG
Hairpin Sequence:
5'-CCGG-ACAGTAATCTGGGCATCATTG-CTCGAG-CAATGATGCCCAGATTACTGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAGTAATCTGGGCATCATTGCTCGAGCAATGATGCCCAGATTACTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAGTAATCTGGGCATCATTGCTCGAGCAATGATGCCCAGATTACTGT-3'

Other clones with same target sequence:

(none)