Construct: shRNA TRCN0000428587

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152629.3-2923s21c1
DNA Barcode:
AGTGACCTGCCTCACCTAATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GLIS3 (169792)
Gene Description:
GLIS family zinc finger 3
Transcript:
RefSeq NM_152629.3 (CURRENT)
Match location:
Position 2923 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000428587)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTGACCTGCCTCACCTAATC
Hairpin Sequence:
5'-CCGG-AGTGACCTGCCTCACCTAATC-CTCGAG-GATTAGGTGAGGCAGGTCACT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTGACCTGCCTCACCTAATCCTCGAGGATTAGGTGAGGCAGGTCACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTGACCTGCCTCACCTAATCCTCGAGGATTAGGTGAGGCAGGTCACT-3'

Other clones with same target sequence:

(none)