Construct: shRNA TRCN0000428654
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_032034.2-1451s21c1
- DNA Barcode:
- GTTTCTTCCTTGCGCTTTATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SLC4A11 (83959)
- Gene Description:
- solute carrier family 4 member 11
- Transcript:
- RefSeq NM_032034.2 (NON-CURRENT)
- Match location:
- Position 1451 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000428654)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GTTTCTTCCTTGCGCTTTATG
- Hairpin Sequence:
-
5'-CCGG-GTTTCTTCCTTGCGCTTTATG-CTCGAG-CATAAAGCGCAAGGAAGAAAC-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGTTTCTTCCTTGCGCTTTATGCTCGAGCATAAAGCGCAAGGAAGAAACTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGTTTCTTCCTTGCGCTTTATGCTCGAGCATAAAGCGCAAGGAAGAAAC-3'
Other clones with same target sequence:
(none)
