Construct: shRNA TRCN0000428703

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198153.2-1177s21c1
DNA Barcode:
GCCCTAGAGTCCTATGAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TREML4 (285852)
Gene Description:
triggering receptor expressed on myeloid cells like 4
Transcript:
RefSeq NM_198153.2 (NON-CURRENT)
Match location:
Position 1177 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000428703)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTAGAGTCCTATGAATTT
Hairpin Sequence:
5'-CCGG-GCCCTAGAGTCCTATGAATTT-CTCGAG-AAATTCATAGGACTCTAGGGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTAGAGTCCTATGAATTTCTCGAGAAATTCATAGGACTCTAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTAGAGTCCTATGAATTTCTCGAGAAATTCATAGGACTCTAGGGC-3'

Other clones with same target sequence:

(none)