Construct: shRNA TRCN0000428720

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_130808.1-1019s21c1
DNA Barcode:
GAACTGAGGTTGTGATGAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CPNE4 (131034)
Gene Description:
copine 4
Transcript:
RefSeq NM_130808.1 (NON-CURRENT)
Match location:
Position 1019 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000428720)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACTGAGGTTGTGATGAATA
Hairpin Sequence:
5'-CCGG-GAACTGAGGTTGTGATGAATA-CTCGAG-TATTCATCACAACCTCAGTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACTGAGGTTGTGATGAATACTCGAGTATTCATCACAACCTCAGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACTGAGGTTGTGATGAATACTCGAGTATTCATCACAACCTCAGTTC-3'

Other clones with same target sequence:

(none)