Construct: shRNA TRCN0000428781

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007583.2-466s21c1
DNA Barcode:
TCGCTGTGGGAACCGACTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cacng2 (12300)
Gene Description:
calcium channel, voltage-dependent, gamma subunit 2
Transcript:
RefSeq NM_007583.2 (CURRENT)
Match location:
Position 466 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000428781)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGCTGTGGGAACCGACTATT
Hairpin Sequence:
5'-CCGG-TCGCTGTGGGAACCGACTATT-CTCGAG-AATAGTCGGTTCCCACAGCGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGCTGTGGGAACCGACTATTCTCGAGAATAGTCGGTTCCCACAGCGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGCTGTGGGAACCGACTATTCTCGAGAATAGTCGGTTCCCACAGCGA-3'

Other clones with same target sequence:

(none)