Construct: shRNA TRCN0000428862

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172366.2-217s21c1
DNA Barcode:
CCCTGCTTGGCAAATGGTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FBXO16 (157574)
Gene Description:
F-box protein 16
Transcript:
RefSeq NM_172366.2 (NON-CURRENT)
Match location:
Position 217 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000428862)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGCTTGGCAAATGGTTTG
Hairpin Sequence:
5'-CCGG-CCCTGCTTGGCAAATGGTTTG-CTCGAG-CAAACCATTTGCCAAGCAGGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGCTTGGCAAATGGTTTGCTCGAGCAAACCATTTGCCAAGCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGCTTGGCAAATGGTTTGCTCGAGCAAACCATTTGCCAAGCAGGG-3'

Other clones with same target sequence:

(none)