Construct: shRNA TRCN0000428899

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175236.4-1031s21c1
DNA Barcode:
TGGCATGTCTTACCCAATTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adhfe1 (76187)
Gene Description:
alcohol dehydrogenase, iron containing, 1
Transcript:
RefSeq NM_175236.4 (CURRENT)
Match location:
Position 1031 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000428899)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGCATGTCTTACCCAATTTC
Hairpin Sequence:
5'-CCGG-TGGCATGTCTTACCCAATTTC-CTCGAG-GAAATTGGGTAAGACATGCCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGCATGTCTTACCCAATTTCCTCGAGGAAATTGGGTAAGACATGCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGCATGTCTTACCCAATTTCCTCGAGGAAATTGGGTAAGACATGCCA-3'

Other clones with same target sequence:

(none)