Construct: shRNA TRCN0000429081
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_001510.2-2302s21c1
- DNA Barcode:
- GTCCTAGACTCTGCGGTATAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GRID2 (2895)
- Gene Description:
- glutamate ionotropic receptor delta type subunit 2
- Transcript:
- RefSeq NM_001510.2 (NON-CURRENT)
- Match location:
- Position 2302 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GTCCTAGACTCTGCGGTATAT
- Hairpin Sequence:
-
5'-CCGG-GTCCTAGACTCTGCGGTATAT-CTCGAG-ATATACCGCAGAGTCTAGGAC-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGTCCTAGACTCTGCGGTATATCTCGAGATATACCGCAGAGTCTAGGACTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGTCCTAGACTCTGCGGTATATCTCGAGATATACCGCAGAGTCTAGGAC-3'
Other clones with same target sequence:
(none)
