Construct: shRNA TRCN0000429088

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007392.2-551s21c1
DNA Barcode:
GTGACTCACAACGTGCCTATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Acta2 (11475)
Gene Description:
actin, alpha 2, smooth muscle, aorta
Transcript:
RefSeq NM_007392.2 (NON-CURRENT)
Match location:
Position 551 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429088)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGACTCACAACGTGCCTATC
Hairpin Sequence:
5'-CCGG-GTGACTCACAACGTGCCTATC-CTCGAG-GATAGGCACGTTGTGAGTCAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGACTCACAACGTGCCTATCCTCGAGGATAGGCACGTTGTGAGTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGACTCACAACGTGCCTATCCTCGAGGATAGGCACGTTGTGAGTCAC-3'

Other clones with same target sequence:

(none)