Construct: shRNA TRCN0000429158

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002535.2-2258s21c1
DNA Barcode:
CCTACTGTAGCAACCTGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OAS2 (4939)
Gene Description:
2'-5'-oligoadenylate synthetase 2
Transcript:
RefSeq NM_002535.2 (NON-CURRENT)
Match location:
Position 2258 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429158)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTACTGTAGCAACCTGAAAT
Hairpin Sequence:
5'-CCGG-CCTACTGTAGCAACCTGAAAT-CTCGAG-ATTTCAGGTTGCTACAGTAGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTACTGTAGCAACCTGAAATCTCGAGATTTCAGGTTGCTACAGTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTACTGTAGCAACCTGAAATCTCGAGATTTCAGGTTGCTACAGTAGG-3'

Other clones with same target sequence:

(none)