Construct: shRNA TRCN0000429171

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001949.3-810s21c1
DNA Barcode:
ACGAAGTCCAGATAGTCCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
E2F3 (1871)
Gene Description:
E2F transcription factor 3
Transcript:
RefSeq NM_001949.3 (NON-CURRENT)
Match location:
Position 810 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429171)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGAAGTCCAGATAGTCCAAA
Hairpin Sequence:
5'-CCGG-ACGAAGTCCAGATAGTCCAAA-CTCGAG-TTTGGACTATCTGGACTTCGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGAAGTCCAGATAGTCCAAACTCGAGTTTGGACTATCTGGACTTCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGAAGTCCAGATAGTCCAAACTCGAGTTTGGACTATCTGGACTTCGT-3'

Other clones with same target sequence:

(none)