Construct: shRNA TRCN0000429255

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003305.2-1969s21c1
DNA Barcode:
ACTTCCGTTGTGCTCAAATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRPC3 (7222)
Gene Description:
transient receptor potential cation channel subfamily C member 3
Transcript:
RefSeq NM_003305.2 (CURRENT)
Match location:
Position 1969 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429255)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTTCCGTTGTGCTCAAATAT
Hairpin Sequence:
5'-CCGG-ACTTCCGTTGTGCTCAAATAT-CTCGAG-ATATTTGAGCACAACGGAAGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTTCCGTTGTGCTCAAATATCTCGAGATATTTGAGCACAACGGAAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTTCCGTTGTGCTCAAATATCTCGAGATATTTGAGCACAACGGAAGT-3'

Other clones with same target sequence:

(none)