Construct: shRNA TRCN0000429285

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153402.2-1009s21c1
DNA Barcode:
GACTGATTCTCATCGGGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ago3 (214150)
Gene Description:
argonaute RISC catalytic subunit 3
Transcript:
RefSeq NM_153402.2 (CURRENT)
Match location:
Position 1009 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429285)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTGATTCTCATCGGGTAAA
Hairpin Sequence:
5'-CCGG-GACTGATTCTCATCGGGTAAA-CTCGAG-TTTACCCGATGAGAATCAGTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTGATTCTCATCGGGTAAACTCGAGTTTACCCGATGAGAATCAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTGATTCTCATCGGGTAAACTCGAGTTTACCCGATGAGAATCAGTC-3'

Other clones with same target sequence:

(none)