Construct: shRNA TRCN0000429290

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003493.2-312s21c1
DNA Barcode:
GTTTGAGGACACCAACCTGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H3-4 (8290)
Gene Description:
H3.4 histone
Transcript:
RefSeq NM_003493.2 (CURRENT)
Match location:
Position 312 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429290)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTTGAGGACACCAACCTGTG
Hairpin Sequence:
5'-CCGG-GTTTGAGGACACCAACCTGTG-CTCGAG-CACAGGTTGGTGTCCTCAAAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTTGAGGACACCAACCTGTGCTCGAGCACAGGTTGGTGTCCTCAAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTTGAGGACACCAACCTGTGCTCGAGCACAGGTTGGTGTCCTCAAAC-3'

Other clones with same target sequence:

(none)